ib biology enzyme ia example

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What is the pH of H2O2 solutions?

J. Biosci. [image online] Available at: http://en.wikipedia.org/wiki/File:Noncompetitive_inhibition.svg [Accessed: 10 Jan 2014] 2 Biology IA: Enzymes and Inhibition 002223-0028 Variables: Independent Variable Concentration of CuSO4 solution 0 mol dm-3 0.2 mol dm-3 0.4 mol dm -3 0.6 mol dm-3 0.8 mol dm-3 1 mol dm-3 1. If the investigation occurs over the course of a few days make sure to mark your apparatus, clear the working area and refrigerate liver and H2O2 each time and the same at the end of the experiment. Apparatus Like pH, temperature has a profound impact on the active sites and high temperatures denature the enzyme because they change the active site irreversibly.

80/1. The experiment was too time consuming, spanned over several days which may have had an impact on the result. 12 Biology IA: Enzymes and Inhibition 002223-0028 Evaluation: The experimental design was suitable for this experiment, but it was very time consuming as initially it was hard to get one method right and the method in this IA was achieved after many preliminary trials. 2. The Study of pH Influence on Bovine Liver Catalase by Means of UV-VIS Spectroscopy and Spin Labelling Method. Apart from inhibitors, pH levels, temperature and substrate concentration also have an impact on enzyme activity. the active site can no longer bind to the substrate; the curve plateaus out. [online] Available at: http://www.123helpme.com/view.asp?id=148488 [Accessed: 12 Jan 2014]. Be sure you know what the terms are, how they have been researched, and. Investigating the Effect of Copper Sulphate on Amylase Activity: Papers. 101 (1): 7376 Ward, W. and Damon, A. Qualitative analysis: Apart from the quantitative analysis above, some other physical changes were observed over the course of this experiment. 4 H2o2.com. "Production of catalase by fungi growing at low pH and high temperature". Remove the cork and empty the contents of the boiling tube in a sink but make sure you use a sieve for filtering out the liver. By continuing well assume youre on board with our cookie policy. It was possible to heat water and use it in a big beaker in which the boiling tube would be immersed. [online] Available at: http://www.elmhurst.edu/~chm/vchembook/573inhibit.html [Accessed: 10 Jan 2014]. [emailprotected] Sites.google.com. But there are certain conditions that need to be fulfilled in order for the enzymes to work. [online] Available at: http://www.h2o2.com/faqs/FaqDetail.aspx?fId=26 [Accessed: 12 Jan 2014]. This is an exception to the onion however as the onion produced an average of 2. Amount of hydrogen peroxide used to test each of the substances| Measured carefully with a measuring cylinder | Quantity of each substance| Each vegetables and fruit (with the exception of the Chinese cabbage) is cut to a block shape of 5?

Processed Data: Sample calculation of mean amount of bubbles produced: Where: Ex = Sum of all values n = Number of Values Where: Ex = Sum of all values n = Number of Values Formula: Mean= ? 14 Bibliography: 123helpme.com. Although the results follow the general pattern that catalase activity decreases as concentration of CuSO4 increases- which can be called an inverse relationship- however it is not proportional or linear per se as there were many differences in the individual results of the trials. | H2O2.com - US Peroxide - Technologies for Clean Environment. PhDessay is an educational resource where over 1,000,000 free essays are collected.

18 T-test of potato and red apple = 27. 1cm. We brought real Experts onto our platform to help you even better! Enzymes and inhibition THE EFFECT OF COPPER, Views 2,808 Handling raw liver may be an unpleasant experience for some; lab coats should be worn in case the student fears it might spill over on his/her clothes. Ronald F. Clayton If there was a stencil that helped cute 0.50 g pieces, that would have saved a lot of time.

As a result, less oxygen will be produced during the breakdown of hydrogen peroxide into oxygen and water the higher the concentration of CuSO4 will be.

Rahway, NJ: Merck and Company ASTDR. A theory to why carrots and potatoes contain more amylase than the other food items, is that both of the plant species belongs to the Asterids clade. The onion, on the other hand produced very little amount of bubble compared to the carrot and producing an average of 2. Consistently less oxygen was produced overall each time the concentration was increased by 0.2 mol dm-3 as can been seen in the mean values 53.2, 44.8, 38.2, 22, 13.2 and 10 and in the downward sloping line of best fit in the graph on the previous page. [online] Available at: http://www.elmhurst.edu/~chm/vchembook/573inhibit.html [Accessed: 10 Jan 2014]. were used. Control of the temperature Improvement(s) One of the most time consuming aspects was weighing the liver to be 0.50 g each time (and overall this was done 30 times). Weakness 1.

2. 1? 1983. Then prepare the different concentrations of CuSO4 using the dilutions method discusses earlier in the Materials section. Overleaf there is a diagrammatic explanation of the mechanism of noncompetitive inhibition.

Let us help you get a good grade on your paper. 1cm potato with 3% hydrogen peroxide, Test tube Test tube Labeled Diagram: Data Collection and Processing Raw Data Table: Table 1: Amount of bubbles released from each test tube Processed Data Table: Table 2: Mean and standard deviation of amount of bubbles released from each test tube Graph: *Error bars represent the uncertainty of the bubble count of the experiment. Drop 2 cm3 of the pH 7 buffer in with the liver while simultaneously starting the stopwatch. [e-book] Katowice: Polish Journal of Environmental Studies. 1? Non-competitive inhibition.

48.5, 61, 45, etc.). Enzyme Inhibitors. 2007.

Concentration of CuSO4/ mol dm-3 Standard Deviation 0 Mean volume of O2 produced/ cm3 53.3 6.48 Total percentage error 2.60% 0.2 45.8 8.96 2.62% 0.4 38.2 11.3 2.63% 0.6 22 2.92 2.74% 0.8 13.2 1.48 2.89% 1 10 2.35 3.01% Table 2: Processed data 10 Biology IA: Enzymes and Inhibition 002223-0028 The effect of CuSO4 on the activity of catalase 70 Mean volume of O2 produced / cm3 60 50 40 30 20 10 0 0 0.2 0.4 0.6 Concentration of CuSO4 / mol 0.8 1 1.2 dm-3 Graph: Mean volumes of O2 produced with respect to the different concetrations of CuSO4 used, the error bars represent standard deviation 11 Biology IA: Enzymes and Inhibition 002223-0028 Conclusion: The results of this experiment seem to agree with the hypothesis that increasing the concentration of CuSO4 will decrease the enzymatic activity of catalase, and that indeed its behavior is characteristic to that of an inhibitor.

The majority of variations in results exist because catalase is not evenly distributed in the liver, and utilizing different portions lead to slightly different results. Use the cutting knife to cut the piece of apple into halves, after that use the knife to cut 5 pieces of the red apples into 5?

Increasing substrate H2O2 was used and a dropping pipette concentration increases the rate of was used to measure 3 cm3 of it every the reaction but after a certain limit, time. These will we discussed further while explaining the controlled variables in this experiment. Design.

The plunger will quickly start moving outwards indicating oxygen production. This is because the Cu2+ in CuSO4, like other metallic ions, acts as an inhibitor1. 60 Standard Deviation of potato = 24. Introduction: Enzymes are globular proteins, they are responsible for most of the chemical activities of a living organism. The equation for the decomposition of H2O2 is: 2 H2O2 2 H2O + O2 Like all enzymes catalase has an active site, the surface of which comes into contact with the substrate. 17 = 27. If you ran out of idea, think of these followings that I have taken some Bio IA topics from previous classmates: 1. Harlow, [England]: Pearson Education. [image online] Available at: http://en.wikipedia.org/wiki/File:Non-competitive_inhibition.svg [Accessed: 10 Jan 2014] Windholz, M., ed. optimum pH for catalase5. pH 4 and pH 10 buffer solutions perhaps because the acidic pH is not were also available but pH 7 was used optimal for the enzyme. Save time and let our verified experts help you. n.d. Endothermic and Exothermic Reactions - LMS Room 225. Discuss pesticide problems relating to beekeeping, including symptoms, distinguishing from disease and preventing pesticide impact. Cut a small piece of it using a scalpel and weigh it on the balance. Malak Ibrahimi Candidate Number:000001 30.10.2015 Exam 2016 If the mass is not 0.50 g, make it reach that value by cutting a little more or using a new slightly bigger piece. (2017, Mar 03). The potato showed a good amount of bubbles produced, with the average of 23.

The syllabus and instructions for IA were unclear, but after one year of "guinea pig" trial on the 2016 people, I am sure your teacher has some first-hand experience in giving you useful comments. Using the pipette dropper to suck in 10 ml of the 3% hydrogen peroxide, drop 10ml of hydrogen peroxide into a test tube.

[online] Available at: https://sites.google.com/a/wvde.k12.wv.us/lms-room-225/endothermic-and-exothermic-reactions [Accessed: 12 Jan 2014]. Enzymes have an optimum pH and higher or lower pH conditions might damage the active site by changing its chemical structure.

Biology Enzymes IA Design Introduction: Enzymes are globular proteins, they are responsible for most of the chemical activities of a living organism. Previously, the 5 formula obtained was 2.508 + %, now the x can be substituted for the mean values of the results of different concentrations. At this point there should be 5 pieces (each) of red apples, carrots, potatoes and onions of a volume of 5? 1? 6. They act as catalysts, substances that affects the reaction of other substances without being destroyed or altered during the process. We use cookies to give you the best experience possible. For example, the average bubble release of the carrot is 56.

6 Biology IA: Enzymes and Inhibition 002223-0028 Procedure: 1. Bioeng. Different food items will produce different amount of bubbles when tested with the hydrogen peroxide Variables: Table of Variables Independent Variable| Dependent Variable| Controlled Variable | Uncontrolled Variables| Different types of vegetables and fruits used for catalase testing| Amount of bubbles produced in the reaction| * Amount of hydrogen peroxide used to test each of the substances * Quantity of each substance * pH of the hydrogen peroxide| Temperature of the room | Table of Controlled Variables Variables | Method of Control |, Order custom essay Ib Biology Enzymes Ia Research Question: In what manner will the product of the enzymatic reaction be released from the different substances placed with the test tube?

Background information: Catalase is a very important enzyme that is found in nearly all organisms to catalyze the decomposition of toxic hydrogen peroxide into harmless water and oxygen. Therefore is important that gloves and safety goggles are worn over the course of the whole experiment to avoid it touching the skin or the eyes which can cause inflammation.

ib biology enzyme ia example
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